| Critical
studies on the effect of growth regulators on in vitro shoot
proliferation in Rosa x hybrida L. cv. Sonia for micropropagation. |
| Singh, S.
K.,Syamal, M. M. |
| Department of
Horticulture, Institute of Agricultural Sciences, Banaras Hindu
University, Varanasi 221005, India. |
| A
micropropagation method for roses cv. Sonia is presented. Shoot
proliferation was best (70.3%) on MS [Murashige and Skoog] medium
supplemented with BAP [benzyladenine] at 2 mg/litre + NAA at 0.1
mg/litre + GA3 at 0.01 mg/litre, with a proliferation of >5
microshoots per subculture. Efficient rooting was achieved on
half-strength MS medium supplemented with IBA at 0.2 mg/litre + NAA at
0.1 mg/litre. Rooted plantlets were acclimatized for 3 weeks and planted
out under field conditions with a survival of over 70%. |
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